Endotoxin is a bacterial residue that survives filtration and freeze-drying and can trigger immune and inflammatory responses in cultured cells on its own. The certificate reports it in endotoxin units per milligram. How the test works, a dilution table for every catalog vial, which cell types care, and when the number matters.
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Every vial in the catalog reconstituted at 1, 2 and 3 mL, with the resulting mg/mL, micrograms per insulin-syringe unit, and the draw for a 250 mcg aliquot. Plus the molar concentration from PubChem weights, and the two vials that need a different approach.
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Two different compounds are sold under the name CJC-1295. One carries a Drug Affinity Complex that binds albumin and acts for days; the other is the bare 29-residue GRF analogue that acts for minutes. What the difference is, how the literature describes each, and how the certificate tells them apart.
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Bacteriostatic water is sterile water with 0.9% benzyl alcohol added to stop microbial growth in a vial drawn from repeatedly. Benzyl alcohol is not inert to cultured cells. What the preservative is for, how much of it reaches a culture at each dilution, when to use sterile water or buffer instead, and how to control for it.
Read the post →The blog covers buying, handling and research-design questions as they come up. The evergreen material lives in the guides: reading a certificate of analysis, storage and stability, one research guide per compound and the glossary.
New posts publish to the RSS feed. Every post is written for laboratory research use only and links to the lot certificate for any product it mentions.
One email when a new certificate is published, when a sold-out compound is back, or when a new compound is added. About twice a month, nothing else.
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