NAD+

What is NAD+?

NAD+ is nicotinamide adenine dinucleotide, the coenzyme at the centre of cellular energy metabolism and a substrate for sirtuins and PARP enzymes. It is not a peptide, but it is supplied here because it sits in the same longevity and cellular-ageing research programmes as the peptides in this category. Pepstral supplies NAD+ as a lyophilized powder, 500 mg per vial, with purity and identity confirmed on every lot.

→Read the full NAD+ research guide

What is NAD+ studied for?

• Sirtuin and PARP enzyme activity assays
• Mitochondrial function and cellular-ageing models
• NAD+/NADH ratio studies
• Combination work with MOTS-c and other mitochondrial peptides

All applications above are in-vitro or preclinical research contexts. NAD+ is sold for laboratory research only and is not for human or animal use.

What does the certificate for lot NAD500-0803 show?

Every lot of NAD+ is tested by an independent US laboratory before it is listed. The report for lot NAD500-0803 is in the COA library and the lot number printed on the vial matches it. The current results:

Test	Method	Lot NAD500-0803 result
Identity	LC-MS/MS	NAD+ · conforms
Content	HPLC quantitation	503.42 mg
Purity	RP-HPLC (214 nm)	99.78%
Endotoxin	USP <85>	<0.05 EU/mL
Heavy metals	USP <232>	<0.01 ppm

How is NAD+ handled and stored?

Sealed vials are stored at -20°C away from light and keep for 24 months. To reconstitute, add bacteriostatic water slowly down the inside wall of the vial and swirl until clear; never shake. Once in solution, keep at 2 to 8°C and use within our 28-day default for a preserved solution. The concentration table gives the dilution volumes and microlitre aliquots for this vial.

Frequently asked questions

Also ordered as part of longevity stack and nad stack.

Related reading

• Humanin: the 24-residue peptide encoded in mitochondrial 16S rRNA, the certificate for lot HUM10-0803, and why one cysteine and four leucines decide its handlingHumanin, 24 residues from mitochondrial 16S rRNA, mass 2687.2. Lot HUM10-0803 line by line, and the handling its cysteine and leucines demand.
• MOTS-c: what the certificate for lot MOTS10-0803 shows, and why two methionines and a tryptophan decide how it is handledMOTS-c, 16 residues from mitochondrial 12S rRNA, mass 2174.6. Lot MOTS10-0803 line by line, and the handling its two methionines demand.
• SS-31 (elamipretide): a four-residue peptide with a D-arginine and a dimethyltyrosine, the certificate for lot SS31-10-0803, and the approval granted in September 2025SS-31 is D-Arg-Dmt-Lys-Phe-NH2, mass 639.8. Lot SS31-10-0803 line by line, handling, and its FDA approval as elamipretide in September 2025.
• Why there is no home purity test kit for peptides: what purity actually requires, what a strip or colour test can detect, and the checks you can do at the benchPurity needs a separation, which no strip or handheld device does. What those tests detect, which bench checks work, and what a certificate replaces.