What is NAD+, and what is it studied for?
NAD+ is nicotinamide adenine dinucleotide, the coenzyme at the centre of cellular energy metabolism and the substrate consumed by sirtuins, PARP enzymes and CD38. It is not a peptide. It is supplied in the longevity category because it is used alongside the peptides there in cellular-ageing, mitochondrial-function and DNA-repair research, and because cellular NAD+ levels decline with age in most tissues studied.
What is the structure of NAD+?
A dinucleotide of adenine and nicotinamide joined through two phosphate groups, with a molecular weight of about 663 daltons. Supplied as a lyophilized powder; identity by LC-MS, purity by HPLC-UV. It cycles between NAD+ and its reduced form NADH in redox reactions.
| Identifier | Value |
|---|---|
| Molecular formula | C21H27N7O14P2 |
| Molecular weight | 663.4 g/mol |
| Residues | not a peptide |
| CAS number | 53-84-9 |
| PubChem CID | 5892 |
| Current lot | NAD500-0803 |
How is NAD+ thought to work?
As a redox cofactor NAD+ carries electrons in glycolysis, the TCA cycle and oxidative phosphorylation. As a substrate it is consumed by sirtuins in deacetylation reactions linked to metabolic regulation and by PARPs in DNA repair. Falling NAD+ with age reduces sirtuin activity, which is the hypothesis behind most NAD+ research; raising it through precursors or direct supply is the intervention being tested.
Which research areas use NAD+?
| Area | Typical models and endpoints |
|---|---|
| Sirtuin and PARP activity | Enzyme assays and cellular readouts of deacetylation and DNA-repair capacity. |
| Mitochondrial function | Respiration, membrane potential and biogenesis in cell models. |
| NAD+/NADH ratio | Redox-state measurement under metabolic stress. |
| Combination with mitochondrial peptides | Co-treatment with MOTS-c and related compounds. |
What does the published literature show?
Reviews by Rajman, Chwalek and Sinclair in Cell Metabolism (2018) and by Covarrubias and colleagues in Nature Reviews Molecular Cell Biology (2021) summarise the decline of NAD+ with age and the preclinical results of restoring it. Human data mostly concern oral precursors rather than NAD+ itself. Research use of the material sold here is in-vitro and preclinical.
How is NAD+ handled in the laboratory?
Sealed at -20°C for 24 months. In solution NAD+ degrades within days even when refrigerated, so prepare fresh for each experiment rather than storing a stock. The concentration table covers dilution volumes and microlitre aliquots; the storage guide covers stability by compound class.
What does the current certificate show?
Lot NAD500-0803 tested at 99.78% purity by RP-HPLC (214 nm), identity confirmed by LC-MS/MS, endotoxin <0.05 EU/mL. The certificate is in the COA library and the lot number on the vial matches it. Sold as 500 mg per vial at $100, for laboratory research only.
Certificate history for NAD+
Every certificate the library holds for this compound, newest first. A replaced lot stays listed so the history can be read; the certificate dataset reads all 114 together.
| Lot | Issued | Size | Identity | Purity | Content | Status |
|---|---|---|---|---|---|---|
| NAD500-0803 | Aug 17, 2026 | 500 mg | LC-MS/MS | 99.78% | 503.42 mg | Current |
| NAD1000-0803 | Aug 17, 2026 | 1000 mg | LC-MS/MS | 99.74% | 1004.23 mg | Current |
| NAD500-0401 | Jun 17, 2026 | 500 mg | MALDI-MS | 99.9% | 502.47 mg | Replaced |
| NAD1000-0401 | Jun 17, 2026 | 1000 mg | MALDI-MS | 99.9% | 1002.51 mg | Replaced |
Frequently asked questions
Is NAD+ a peptide?
No. It is a dinucleotide coenzyme, listed here because it sits in the same research programmes as the longevity peptides.
What does lot NAD500-0803 report?
99.78% purity by RP-HPLC (214 nm), identity NAD+ confirmed by LC-MS/MS, content 503.42 mg by HPLC quantitation, endotoxin <0.05 EU/mL, heavy metals <0.01 ppm. Certificate COA7592, issued 17 August 2026 by Bioviridian Inc.
Why prepare it fresh?
NAD+ hydrolyses in aqueous solution over days, so a stored stock loses potency; make what the experiment needs.
References
- Rajman L, Chwalek K, Sinclair DA. Therapeutic potential of NAD-boosting molecules: the in vivo evidence. Cell Metab. 2018.
- Covarrubias AJ et al. NAD+ metabolism and its roles in cellular processes during ageing. Nat Rev Mol Cell Biol. 2021.
Clinical results cited above describe regulated trials of pharmaceutical products and are given as context for research interest only. Nothing on this page describes or recommends use of the material sold here in humans or animals.
NAD+ 500 mg
$100 per vial
Lot NAD500-0803 · 99.78% RP-HPLC (214 nm) · certificate published
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