
Bacteriostatic water and benzyl alcohol: when the preservative gets into your cell work
Bacteriostatic water is sterile water with 0.9% benzyl alcohol added to stop microbial growth in a vial drawn from repeatedly. Benzyl alcohol is not inert to cultured cells. What the preservative is for, how much of it reaches a culture at each dilution, when to use sterile water or buffer instead, and how to control for it.
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