What is TB-500, and what is it studied for?

What is the structure of TB-500?

The fragment corresponds to the actin-binding region of thymosin beta-4, centred on the LKKTET motif, supplied lyophilized as the acetate salt. Because it is a fragment rather than the full protein, studies that require complete thymosin beta-4 should specify the full-length molecule. Identity by LC-MS and purity by HPLC-UV on every lot.

Identifier	Value
Molecular formula	C38H68N10O14
Molecular weight	889.0 g/mol
Residues	7
CAS number	885340-08-9
PubChem CID	62707662
Current lot	not recorded

How is TB-500 thought to work?

Thymosin beta-4 sequesters G-actin, regulating the pool available for polymerisation and therefore cell motility. It has been reported to promote endothelial and keratinocyte migration, angiogenesis, and to reduce inflammation in injury models. The active fragment reproduces the actin-binding activity in vitro.

Which research areas use TB-500?

Area	Typical models and endpoints
Actin dynamics	Actin-binding and polymerisation assays; cytoskeletal imaging.
Wound healing	Dermal wound closure, keratinocyte migration, corneal injury models.
Angiogenesis	Endothelial tube formation and vessel-density endpoints.
Cardiac and skeletal muscle injury	Post-injury recovery models in rodents.

What does the published literature show?

Thymosin beta-4 has a substantial literature, including reviews by Goldstein, Hannappel and Kleinman describing its actin-sequestering role and repair-related activity, and studies of cardiac repair after injury. Work on the isolated fragment is smaller and mostly preclinical. There are no completed human trials of TB-500 as sold for research.

How is TB-500 handled in the laboratory?

Dissolves readily. Standard reconstitution, refrigerate at 2 to 8°C, 28-day window. Avoid freeze-thaw cycles. The concentration table covers dilution volumes and microlitre aliquots; the storage guide covers stability by compound class.

What does the current certificate show?

Lot TB10-0803 tested at 99.68% purity by RP-HPLC (214 nm), identity confirmed by LC-MS/MS, endotoxin <0.05 EU/mL. The certificate is in the COA library and the lot number on the vial matches it. Sold as 5 mg per vial at $55, for laboratory research only.

Certificate history for TB-500

Every certificate the library holds for this compound, newest first. A replaced lot stays listed so the history can be read; the certificate dataset reads all 114 together.

Frequently asked questions

References

1. Goldstein AL, Hannappel E, Kleinman HK. Thymosin beta4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol Med. 2005.
2. Malinda KM et al. Thymosin beta4 accelerates wound healing. J Invest Dermatol. 1999.
3. Bock-Marquette I et al. Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair. Nature. 2004.

Clinical results cited above describe regulated trials of pharmaceutical products and are given as context for research interest only. Nothing on this page describes or recommends use of the material sold here in humans or animals.